EXPERIMENT_TITLE: GSM7918226: LNCaP_Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Hi-C experiment was conducted using Mbo I, according to the protocol published by Suhas S.P. Rao et al. Cell 2014 (PMID: 25497547). After proximity ligation and fragmentation by COVARIS S220, the sequencing library was generated from fragmented DNA using KAPA HyperPrep Kits following the manufacturer's instructions.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: To elucidate the regulation of NSD2 in metastatic castration-resistant prostate cancer(CRPC), we performed Hi-C against castration-sensitive prostate cancer cell line LNCaP and metastatic castration-resistant prostate cancer cell lines, PC3 respectively. In metastatic CRPC, we found specific regions of activation with epigenetic changes. Overall design: Hi-C seq for prostate cancer cell lines
SAMPLE_TITLE: LNCaP_Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "LNCaP"}, {"TAG": "cell line", "VALUE": "LNCaP"}, {"TAG": "cell type", "VALUE": "prostate cancer"}, {"TAG": "geo_loc_name", "VALUE": "missing"}, {"TAG": "collection_date", "VALUE": "missing"}]
Pool_Member_@attributes_sample_title: LNCaP_Hi-C
RUN_SET_RUN_Pool_Member_@attributes_sample_title: LNCaP_Hi-C
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