EXPERIMENT_TITLE: GSM2428900: DS23107; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Hi-C experiment were conducted according to previous publication (Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326, 289-93 (2009).) Libraries were prepared according to Illumina's protocol for Truseq library construction, with the following modifications:  Volumes and primers were scaled down to accommodate low input material.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Utilizing HiC to characterize the three-dimensional architecture of two NSCLC lines: NCIH460A, NCIH460B. Overall design: Cell lines are cultured in accordance with supplier protocols and assayed following the Dekker Lab''s HiC Assay protocol: http://www.jove.com/video/1869/hi-c-a-method-to-study-the-three-dimensional-architecture-of-genomes. http://www.jove.com/video/1869/hi-c-a-method-to-study-the-three-dimensional-architecture-of-genomes
SAMPLE_TITLE: DS23107
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "immortalized cell line"}, {"TAG": "cell line", "VALUE": "NCIH460A"}, {"TAG": "cell type", "VALUE": "Human large cell lung carcinoma"}, {"TAG": "restriction enzyme", "VALUE": "HindIII"}]
Pool_Member_@attributes_sample_title: DS23107
RUN_SET_RUN_Pool_Member_@attributes_sample_title: DS23107
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR5110346", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR5110346/SRR5110346", "size": "9153919275", "date": "2016-12-14 14:05:49", "md5": "be5174761a873bf90aeaf0560b904358", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR5110346.sralite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos5/sra-pub-zq-14/SRR005/110/SRR5110346.sralite.1", "size": "4074672537", "date": "2020-06-21 16:52:47", "md5": "9238a00009d30c2935a9eb2324b8b2b4", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]