EXPERIMENT_TITLE: Hi-C of U266-CSCs: control
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We establish a method to reprogram bulk multiple myeloma (MM) cancer cells into MM cancer stem cells (MMSCs) via heterochromatin modulation, which allows us to obtain sufficient functional MMSCs in an efficient manner. Characterization of these induced MMSCs demonstrates that the reprogrammed MMSCs possess the properties of cancer stem cells both phenotypically and functionally. To leverage this model therapeutically, we screened a panel of compounds, and identified dihydroartemisinin (DHA) as a potent anti-MMSCs drug both in vitro and in vivo. DHA induces anti-proliferation effects on MMSCs via nuclear lamina perturbation and chromatin structure alteration. Hi-C analysis reveals that DHA modulates chromatin architecture mainly at the compartmental level and TADs (topologically associating domains) level. The potent anti-MMSCs efficacy and the novel molecular insights establish the therapeutic rationale for DHA in overcoming therapy resistance caused by cancer stem cells.
SAMPLE_TITLE: U266 CSC-control
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "isolate", "VALUE": "not collected"}, {"TAG": "age", "VALUE": "not collected"}, {"TAG": "biomaterial_provider", "VALUE": "ATCC"}, {"TAG": "sex", "VALUE": "not collected"}, {"TAG": "tissue", "VALUE": "blood"}, {"TAG": "cell_line", "VALUE": "U266-CSCs"}, {"TAG": "cell_type", "VALUE": "Multiple myeloma cancer stem cells"}, {"TAG": "disease", "VALUE": "Multiple myeloma"}, {"TAG": "population", "VALUE": "human"}, {"TAG": "treatment", "VALUE": "untreated"}, {"TAG": "BioSampleModel", "VALUE": "Human"}]
Pool_Member_@attributes_sample_title: U266 CSC-control
RUN_SET_RUN_Pool_Member_@attributes_sample_title: U266 CSC-control
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