EXPERIMENT_TITLE: GSM2386378: K562; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Follow DLO Hi-C library construction protocol. Cells were double cross-linked with EGS and formaldehyde and then digested with a restriction enzyme. The digested chromatins were divided into two tubes, ligated with different 20-bp half linkers by the simultaneous digestion and ligation, and subsequently mixed for in-gel proximity ligation. The ligated DNA was purified and digested by MmeI, which released specific 80-bp DLO Hi-C DNA fragments. These DLO Hi-C DNA fragments were ligated with Illumina adapters and subjected to high-throughput sequencing
STUDY_DESCRIPTOR_STUDY_ABSTRACT: we developed a simple Digestion-Ligation-Only Hi-C (DLO Hi-C) technology to explore the 3D landscape of the genome Overall design: The DLO Hi-C library of K562 and THP-1
SAMPLE_TITLE: K562
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "human erythroleukemic cell line"}, {"TAG": "tissue", "VALUE": "human erythroleukemic cell line"}, {"TAG": "cell line", "VALUE": "K562"}]
Pool_Member_@attributes_sample_title: K562
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