EXPERIMENT_TITLE: GSM3304264: HIC_library_Conrtol_replicate2; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Hi-C was performed in two biological replicates using the DpnII restriction endonuclease according to a previously published protocol(Lieberman-Aiden Et al. 2009 (PMID: 19815776);Belton Et al. 2012(PMID: 22652625 )).Each Hi-C library was sequenced using paired end sequencing on an Illumina HiSeq 3000 in two technical replicates;
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We have recently discovered a class of anti-cancer agents, curaxins, which suppress transcription of oncogenes. Here we demonstrate that curaxins preferentially downregulate expression of genes controlled by enhancers and super-enhancers via interrupting enhancer/promoter spatial communication. Our observations made both on a model of enhancer-regulated transcription of chromatinized template and on cultured cancer cells allow classifying curaxins as a novel type of epigenetic drugs that target the 3D genome organization. Overall design: Processed and raw HiC data for control and CBL0137-treated HT1080 cells.
SAMPLE_TITLE: HIC_library_Conrtol_replicate2
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "connective tissue"}, {"TAG": "tissue", "VALUE": "HT1080 cells"}, {"TAG": "disease", "VALUE": "fibrosarcoma"}, {"TAG": "oncogene", "VALUE": "ras +"}]
Pool_Member_@attributes_sample_title: HIC_library_Conrtol_replicate2
RUN_SET_RUN_Pool_Member_@attributes_sample_title: HIC_library_Conrtol_replicate2
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