EXPERIMENT_TITLE: GSM7666392: Jurkat T cells 1; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Briefly, after the completion crosslinking and lysis, chromatin was digested with 200U DPN II restriction enzyme (Qiagen) at 37 °C. DNA fragments were purified and dissolved in water. Unligated ends were then removed. Purified DNA was fragmented to a size of 350-500 bp, and DNA ends were then repaired. DNA fragments labeled by biotin were finally separated on Dynabeads® M-280 Streptavidin (Life Technologies).
STUDY_DESCRIPTOR_STUDY_ABSTRACT: To investigated how Matr3 KO affected the function of CD4 T cells We then performed gene expression profiling analysis using data obtained from Hi-C seq of Jurkat T cells knocked out of Matr3 or not. Overall design: Comparative gene expression profiling analysis of Hi-C seq data for Jurkat T cells knocked out of Matr3 or not.
SAMPLE_TITLE: Jurkat T cells 1
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Jurkat T cells"}, {"TAG": "cell line", "VALUE": "Jurkat T cells"}, {"TAG": "cell type", "VALUE": "T cell"}, {"TAG": "genotype", "VALUE": "WT"}, {"TAG": "geo_loc_name", "VALUE": "missing"}, {"TAG": "collection_date", "VALUE": "missing"}]
Pool_Member_@attributes_sample_title: Jurkat T cells 1
RUN_SET_RUN_Pool_Member_@attributes_sample_title: Jurkat T cells 1
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