EXPERIMENT_TITLE: GSM3791772: NSD2_Low_2_Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We analyzed the impact of NSD2 overexpression on 3D genome reorganization and gene expression in multiple myeloma cell lines Overall design: RNAseq, ChIP-seq, Hi-C and HiChIP in multiple myeloma cell lines
SAMPLE_TITLE: NSD2_Low_2_Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "TKO"}, {"TAG": "parental cell line", "VALUE": "KMS14"}, {"TAG": "cell type", "VALUE": "multiple myeloma cell line"}, {"TAG": "genotype/variation", "VALUE": "overexpressed NSD2 allele inactivated"}, {"TAG": "kit", "VALUE": "Arima"}]
Pool_Member_@attributes_sample_title: NSD2_Low_2_Hi-C
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