EXPERIMENT_TITLE: GSM3211391: Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Five million cells were crosslinked with 1% formaldehyde for 10 min at room temperature, then digested with 125 units of MboI, and labeled by biotinylated nucleotides and proximity-ligated. After reverse crosslinking, ligated DNA was purified and sheared to 300-500 bp, then ligation junctions were pulled down with streptavidin beads and prepared as general Illumina library. The Hi-C sample underwent 76-cycles paired-end sequencing on the Illumina HiSeq 4000.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: In Nalm6, mono-allelic interaction between IGH enhancer and IGH promoter was found from Hi-C data. Overall design: Hi-C
SAMPLE_TITLE: Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "B cell precursor leukemia"}, {"TAG": "cell line", "VALUE": "Nalm6"}, {"TAG": "cell type", "VALUE": "B cell precursor leukemia cell line"}]
Pool_Member_@attributes_sample_title: Hi-C
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