EXPERIMENT_TITLE: GSM6560961: Kidney Medulla-Hi-C-Donor4; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Approximately 0.5 cm^3 of tissue was minced with a razor blade and fixed and nutated for 20 minutes in 10 mls of a 1:10 dilution of 10% neutral buffered formalin (Fisher). Following this, the formalin was quenched by direct addition of 0.1 g of glycine (Sigma) and nutation for another 15 minutes. The tissue fragments were submitted to Phase Genomics for Hi-C library preparation using their Human Hi-C Kit and sequencing on an Illumina HiSeq 4000.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We report chromatin conformation (Hi-C) maps from macro-dissected human kidney cortex and medulla from 1 individual Overall design: Hi-C on macro-dissected kidney cortex and medulla from 1 individual
SAMPLE_TITLE: Kidney Medulla-Hi-C-Donor4
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Kidney Medulla"}, {"TAG": "tissue", "VALUE": "Kidney Medulla"}, {"TAG": "donor", "VALUE": "4"}, {"TAG": "geo_loc_name", "VALUE": "missing"}, {"TAG": "collection_date", "VALUE": "missing"}]
Pool_Member_@attributes_sample_title: Kidney Medulla-Hi-C-Donor4
RUN_SET_RUN_Pool_Member_@attributes_sample_title: Kidney Medulla-Hi-C-Donor4
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR21487013.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR021/21487/SRR21487013/SRR21487013.lite.1", "size": "27523856420", "date": "2022-10-21 21:05:55", "md5": "0f7b539c969204ff6ba3e47eddd60278", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR21487013", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR21487013/SRR21487013", "size": "47537674252", "date": "2022-09-12 01:52:43", "md5": "08089e55603493afae8b0eaede6d4e6c", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Kidney_Akilesh1_Donor4_medulla_R2.fastq.gz", "size": "32914624427", "date": "2022-09-11 23:37:52", "md5": "47d5ef0ad88020ea73247ce2372ea521", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Kidney_Akilesh1_Donor4_medulla_R1.fastq.gz", "size": "30202956625", "date": "2022-09-11 19:34:23", "md5": "15e08b5a78009f0759b3a7deb141f62f", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}]