EXPERIMENT_TITLE: GSM4198772: BLaER subclone, 168h_CTCF_AID_DMSO_HiC_rep2; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Cells were collected by centrifugation. Crosslinked nuclei were digested with MboI followed by fill-in with bio-dATP, in-situ ligation and DNA extraction. DNA was sonicated and libraries were constructed using the the NEBNext DNA Library Prep Kit (New England Biolabs).
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Studies of genome topology, chromatin state and gene expression dynamic during human B cell to macrophage transdifferentiation with and without CTCF Overall design: Genome topology profiling during  human B cell to macrophage transdifferentiation with and without CTCF using insitu HiC
SAMPLE_TITLE: BLaER subclone, 168h_CTCF_AID_DMSO_HiC_rep2
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Leukemia cell line"}, {"TAG": "subclone", "VALUE": "BLaER subclone CTCF-AID (Auxin Inducible Degron)"}, {"TAG": "treatment", "VALUE": "CEBPA+auxin"}, {"TAG": "time", "VALUE": "168h"}]
Pool_Member_@attributes_sample_title: BLaER subclone, 168h_CTCF_AID_DMSO_HiC_rep2
RUN_SET_RUN_Pool_Member_@attributes_sample_title: BLaER subclone, 168h_CTCF_AID_DMSO_HiC_rep2
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "bfeaea1fc_8b15391e7_read1.fastq.gz", "size": "14697994127", "date": "2019-12-01 09:35:04", "md5": "da73c067be8bfe56d31092ea6accc0a2", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "bfeaea1fc_8b15391e7_read2.fastq.gz", "size": "15186849266", "date": "2019-12-01 09:41:15", "md5": "c6661bfd518a3b3455d9ecfa2abdf68d", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR10566634", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR10566634/SRR10566634", "size": "19438347668", "date": "2019-12-01 09:58:08", "md5": "f1813d5d8ef9142b333c31033bfbe862", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR10566634.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos5/sra-pub-zq-14/SRR010/10566/SRR10566634/SRR10566634.lite.1", "size": "9332296646", "date": "2022-06-11 07:06:11", "md5": "496bae93ec4e7c3588179aec959ebb51", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]