EXPERIMENT_TITLE: GSM6560960: Kidney Cortex-Hi-C-Donor4; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Approximately 0.5 cm^3 of tissue was minced with a razor blade and fixed and nutated for 20 minutes in 10 mls of a 1:10 dilution of 10% neutral buffered formalin (Fisher). Following this, the formalin was quenched by direct addition of 0.1 g of glycine (Sigma) and nutation for another 15 minutes. The tissue fragments were submitted to Phase Genomics for Hi-C library preparation using their Human Hi-C Kit and sequencing on an Illumina HiSeq 4000.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We report chromatin conformation (Hi-C) maps from macro-dissected human kidney cortex and medulla from 1 individual Overall design: Hi-C on macro-dissected kidney cortex and medulla from 1 individual
SAMPLE_TITLE: Kidney Cortex-Hi-C-Donor4
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Kidney Cortex"}, {"TAG": "tissue", "VALUE": "Kidney Cortex"}, {"TAG": "donor", "VALUE": "4"}, {"TAG": "geo_loc_name", "VALUE": "missing"}, {"TAG": "collection_date", "VALUE": "missing"}]
Pool_Member_@attributes_sample_title: Kidney Cortex-Hi-C-Donor4
RUN_SET_RUN_Pool_Member_@attributes_sample_title: Kidney Cortex-Hi-C-Donor4
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR21487014.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos2/sra-pub-zq-18/SRR021/21487/SRR21487014/SRR21487014.lite.1", "size": "27530161711", "date": "2022-10-24 21:47:30", "md5": "95d19b65781fa16198cbabb09270eba3", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR21487014", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR21487014/SRR21487014", "size": "48167420851", "date": "2022-09-12 02:24:16", "md5": "9e74a1f9f119239b5baab9a6f6ffeae0", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Kidney_Akilesh2_Donor4_cortex_R2.fastq.gz", "size": "33613162745", "date": "2022-09-12 00:08:50", "md5": "32280a6787eebf55dd4f8073e1cf0c6c", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Kidney_Akilesh2_Donor4_cortex_R1.fastq.gz", "size": "30446938518", "date": "2022-09-12 01:18:16", "md5": "058869870c0bf9711d3c43eb5eb24ee0", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}]