EXPERIMENT_TITLE: GSM5692140: HiC sample replicate 2; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: The Arima-HiC Kit (Arima Genomics, A510008) was used following manufacturer's protocol for low input crosslinking Libraries were prepared with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, 21024). ). Libraries were sequenced on a NovaSeq 6000 (Illumina) in paired-end mode by the Sylvester Comprehensive Cancer Center Onco-Genomics Shared Resource.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: We performed a Hi-C experiment on CD34+ cells isolated from peripheral blood and identifed topologically associated domains (TADs). Overall design: Two replicates of mobilized peripheral blood CD34+ cells were purchased from Fred Hutchinson Hematopoietic Cell Procurement Services. Hi-C was performed in replicate on CD34+ cells.
SAMPLE_TITLE: HiC sample replicate 2
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Peripheral blood CD34+ cells"}, {"TAG": "tissue", "VALUE": "peripheral blood"}, {"TAG": "marker", "VALUE": "CD34 positive"}]
Pool_Member_@attributes_sample_title: HiC sample replicate 2
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