EXPERIMENT_TITLE: HiSeq X Ten paired end sequencing of SAMD00211660
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Aging is one of the risk factors for cancer development because the accumulation of senescent cells during the aging process contributes to various age-related pathologies through senescence-associated secretory phenotype (SASP). Therefore, an understanding of the molecular mechanism involved in SASP regulation could have a tremendous impact on our health. Here, we discovered that noncoding RNA derived from normally silenced pericentromeric repetitive sequences during cellular senescence, and analyzed the effect of the RNA on gene expression and chromosomal alteration in SVts8 cells, a conditionally immortalized human fibroblast cell line.
SAMPLE_TITLE: HiC-hSATII
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "sample_name", "VALUE": "HiC-hSATII"}, {"TAG": "cell_line", "VALUE": "SVts8"}, {"TAG": "cell_type", "VALUE": "Fibroblasts"}, {"TAG": "growth_condition", "VALUE": "SVts8 cells were cultured in DME medium (Nacalai tesque) supplemented with 10% FBS and penicillin/streptomycin (Sigma-Aldrich) at 5% CO2 condition."}]
Pool_Member_@attributes_sample_title: HiC-hSATII
RUN_SET_RUN_Pool_Member_@attributes_sample_title: HiC-hSATII
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "DRR215908", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR215908/DRR215908", "size": "54407055355", "date": "2022-03-19 17:10:52", "md5": "d22b905306acf9648cef3a79cfa6a552", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "DRR215908.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos2/sra-pub-zq-18/DRR000/215/DRR215908/DRR215908.lite.1", "size": "32292381655", "date": "2022-10-19 17:27:15", "md5": "c23b08ca7018f3c1ad5981a3eb208ecc", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]