EXPERIMENT_TITLE: GSM4712759: GSC1517_Control_KD_rep1_Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: BL-Hi-C library construction were performed as described previously (Liang et al., 2017). PCR products between 300–700 bp were purified as BL-Hi-C library using Ampure XP beads and subjected to Illumina HiSeq X10 for paired end 150 bp sequencing.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Bridge Linker-Hi-C (BL-Hi-C) analysis after YY1 knockdown in glioblastoma stem cells Overall design: Bridge Linker-Hi-C (BL-Hi-C) analysis of chromatin interactions after YY1 knockdown in one glioblastoma stem cell (GSC1517)
SAMPLE_TITLE: GSC1517_Control_KD_rep1_Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "patient-derived cells"}, {"TAG": "tissue", "VALUE": "glioblastoma stem cells"}, {"TAG": "cell type", "VALUE": "GSC1517"}, {"TAG": "treatment", "VALUE": "shCONT"}]
Pool_Member_@attributes_sample_title: GSC1517_Control_KD_rep1_Hi-C
RUN_SET_RUN_Pool_Member_@attributes_sample_title: GSC1517_Control_KD_rep1_Hi-C
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Control_KD_rep1_R1.fq.gz", "size": "9145011227", "date": "2020-08-06 17:39:48", "md5": "1c20a43ea7762e4030a51643e428d870", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "Control_KD_rep1_R2.fq.gz", "size": "10236463070", "date": "2020-08-06 17:43:24", "md5": "6589204132512fe35c35bd7d358250e9", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR12403817", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR12403817/SRR12403817", "size": "12885905646", "date": "2020-08-06 17:52:51", "md5": "32446f95f881a3ec540921665df714c4", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR12403817.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos2/sra-pub-zq-18/SRR012/12403/SRR12403817/SRR12403817.lite.1", "size": "8737135366", "date": "2022-07-27 07:18:21", "md5": "c9ff12eb23bb73104328054934ba4cb4", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]