EXPERIMENT_TITLE: GSM5538493: H9 hESC shDDX18; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Human embryonic cells were washed with PBS and fixed with 2% formaldehyde for 10min at RT. Hi-C samples were prepared with the Arima Hi-C Kit, and libraries were preapred with the KAPA HyperPrep Kits following the manufacturer's instructions
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Pluripotent stem cells are unique in their large nucleus and manifestly open chromatin structure with hypertranscriptional activities. How the nucleolus, the largest membraneless and phase-separated subnuclear organelle, maintains its structural integrity in pluripotency maintenance remains incompletely understood. By studying nucleolus-specific DEAD-box RNA helicase-18 (DDX18) in human embryonic stem cells, we discover mechanisms controlling nucleolus phase separation and chromatin organization. Overall design: In this experiment, we characterized hESCchromatin interaction under control and DDX18 knockdown condition using  Hi-C seq.
SAMPLE_TITLE: H9 hESC shDDX18
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