EXPERIMENT_TITLE: GSM5606799: A673-EWS/FLI-Endo-HiC 2; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: AMPure XP bead extraction in situ Hi-C (high throughput chromatin conformation capture)
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Ewing sarcoma is a prototypical fusion transcription factor-associated pediatric cancer that expresses EWS/FLI or highly related fusions. EWS/FLI dysregulates transcription to induce and maintain sarcomagenesis, but the mechanisms utilized are not fully understood. We therefore sought to define the global effects of EWS/FLI on chromatin conformation and transcription in Ewing sarcoma.  We found that EWS/FLI (and EWS/ERG) genomic localization is largely conserved across multiple patient-derived Ewing sarcoma cell lines. EWS/FLI binding is primarily associated with compartment activation, establishment of topologically-associated domain (TAD) boundaries, enhancer-promoter looping that involve both intra- and inter-TAD interactions, and gene activation.  Importantly, local chromatin features provide the basis for transcriptional heterogeneity in regulation of direct EWS/FLI target genes across different Ewing sarcoma cell lines.  These data demonstrate a key role of EWS/FLI in mediating genome-wide changes in chromatin configuration and support the notion that fusion transcription factors serve as master regulators through three-dimensional reprogramming of chromatin. Overall design: in situ Hi-C of A673 Ewing sarcoma cells transduced with shRNA targeting EWS/FLI or Luciferase followed by rescue of EWS/FLI expression with a 3X-FLAG tagged EWS/FLI cDNA construct or empty vector
SAMPLE_TITLE: A673-EWS/FLI-Endo-HiC 2
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "A673 Ewing sarcoma cell line"}, {"TAG": "replicate", "VALUE": "2"}, {"TAG": "transduced shrna", "VALUE": "iLuc + empty cDNA"}, {"TAG": "condition", "VALUE": "EF-Endo"}]
Pool_Member_@attributes_sample_title: A673-EWS/FLI-Endo-HiC 2
RUN_SET_RUN: [{"IDENTIFIERS": {"PRIMARY_ID": "SRR16134759", "SUBMITTER_ID": "GSM5606799_r1"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "38665872", "bases": "11634437395", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L001_R1_001.fastq.gz", "size": "3483029715", "date": "2021-09-30 21:52:09", "md5": "a7985f9854ab58e57d984cb9d90d34f0", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L001_R2_001.fastq.gz", "size": "4106993712", "date": "2021-09-30 21:53:37", "md5": "91f724265296e776e7d97d4aac03a84a", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134759", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134759/SRR16134759", "size": "5323834620", "date": "2021-09-30 21:57:14", "md5": "95d1ebe2966fb1fff0087b2ac1c8ee12", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134759.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134759/SRR16134759.lite.1", "size": "3002894224", "date": "2022-10-04 16:13:07", "md5": "d423019bac88a797905c07ee38266056", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "38665872"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "11634437395"}}, "@attributes": {"alias": "GSM5606799_r1", "accession": "SRR16134759", "total_spots": "38665872", "total_bases": "11634437395", "size": "5323832123", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134760", "SUBMITTER_ID": "GSM5606799_r2"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "40196940", "bases": "12094991578", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L002_R1_001.fastq.gz", "size": "3641957360", "date": "2021-09-30 22:26:36", "md5": "53884de61e1ae9b63b997bcb0fc64693", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L002_R2_001.fastq.gz", "size": "4212386254", "date": "2021-09-30 22:27:55", "md5": "ba3d0b717e84cd7b79d3c585cec61b73", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134760", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134760/SRR16134760", "size": "5505830579", "date": "2021-09-30 22:30:15", "md5": "f2a63646116d90cd994beecd9a4875bb", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134760.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134760/SRR16134760.lite.1", "size": "3121123379", "date": "2022-09-26 10:55:18", "md5": "b8ea43e2a3c150f32c04b764fa18ae36", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "40196940"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "12094991578"}}, "@attributes": {"alias": "GSM5606799_r2", "accession": "SRR16134760", "total_spots": "40196940", "total_bases": "12094991578", "size": "5505828079", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134761", "SUBMITTER_ID": "GSM5606799_r3"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "40351436", "bases": "12141504210", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L003_R1_001.fastq.gz", "size": "3667077306", "date": "2021-09-30 22:53:18", "md5": "4fb1fa8e6425c29a31015f6b99cffc23", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L003_R2_001.fastq.gz", "size": "4210286099", "date": "2021-09-30 22:44:55", "md5": "d19124b5c62f46dec224caddbfaff66e", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134761", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134761/SRR16134761", "size": "5515620779", "date": "2021-09-30 23:01:40", "md5": "24dc4817dc85d66bde96aba5deebd326", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134761.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134761/SRR16134761.lite.1", "size": "3132860035", "date": "2022-09-26 10:55:30", "md5": "93aa4c87495d6e56dedfeab11b0edfba", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "40351436"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "12141504210"}}, "@attributes": {"alias": "GSM5606799_r3", "accession": "SRR16134761", "total_spots": "40351436", "total_bases": "12141504210", "size": "5515618281", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134762", "SUBMITTER_ID": "GSM5606799_r4"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "40145339", "bases": "12079392167", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L004_R1_001.fastq.gz", "size": "3633725555", "date": "2021-09-30 22:22:35", "md5": "efdd0c54683296f9399e0d98f4ad7f3d", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L004_R2_001.fastq.gz", "size": "4178651173", "date": "2021-09-30 22:24:17", "md5": "3162dadd350372efd739a53d486a296e", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134762", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134762/SRR16134762", "size": "5470401841", "date": "2021-09-30 22:27:27", "md5": "6d90d27550267f6af568f72587cc3200", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134762.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134762/SRR16134762.lite.1", "size": "3116747661", "date": "2022-09-26 10:55:44", "md5": "92649fddb500ca4fe024b8dc3c59b9bf", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "40145339"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "12079392167"}}, "@attributes": {"alias": "GSM5606799_r4", "accession": "SRR16134762", "total_spots": "40145339", "total_bases": "12079392167", "size": "5470399339", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134763", "SUBMITTER_ID": "GSM5606799_r5"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "39275227", "bases": "11817663770", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L005_R1_001.fastq.gz", "size": "3558963601", "date": "2021-09-30 22:11:20", "md5": "5aefcba313d3f8ed76a98c63aaa2b3c4", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L005_R2_001.fastq.gz", "size": "4113340749", "date": "2021-09-30 22:09:46", "md5": "a3594c934de5ed3fed78869d8c07b3d3", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134763", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134763/SRR16134763", "size": "5367915953", "date": "2021-09-30 22:14:46", "md5": "318f7bfd4fe87140d23ed7fd453aeae3", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134763.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134763/SRR16134763.lite.1", "size": "3056454129", "date": "2022-09-26 10:58:46", "md5": "9421326d9fdfe51421364224e99b6952", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "39275227"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "11817663770"}}, "@attributes": {"alias": "GSM5606799_r5", "accession": "SRR16134763", "total_spots": "39275227", "total_bases": "11817663770", "size": "5367913452", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134764", "SUBMITTER_ID": "GSM5606799_r6"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "40208635", "bases": "12098529039", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L006_R1_001.fastq.gz", "size": "3632556763", "date": "2021-09-30 21:32:52", "md5": "83434ab59268829ad752aafca2ef4cee", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L006_R2_001.fastq.gz", "size": "4176383215", "date": "2021-09-30 21:33:13", "md5": "9eb9b555245e64c081ee67e2671caba7", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134764", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134764/SRR16134764", "size": "5464458485", "date": "2021-09-30 21:34:16", "md5": "7701ac04a2c6a2491f34a2e655c64ee2", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134764.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134764/SRR16134764.lite.1", "size": "3122050557", "date": "2022-09-26 10:57:50", "md5": "01de501edf140837b544fa6db82d09e5", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "40208635"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "12098529039"}}, "@attributes": {"alias": "GSM5606799_r6", "accession": "SRR16134764", "total_spots": "40208635", "total_bases": "12098529039", "size": "5464455986", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134765", "SUBMITTER_ID": "GSM5606799_r7"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "39656986", "bases": "11932801976", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L007_R1_001.fastq.gz", "size": "3594152180", "date": "2021-09-30 22:11:37", "md5": "b25ffb92b9e55721aa62784da8487be2", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L007_R2_001.fastq.gz", "size": "4144479873", "date": "2021-09-30 22:13:33", "md5": "20335af0cddc8fc4ed217e3207e7df7f", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134765", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134765/SRR16134765", "size": "5416861529", "date": "2021-09-30 22:16:40", "md5": "23bcefa8128e44bb4651208e5efa2453", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134765.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos3/sra-pub-zq-24/SRR016/16134/SRR16134765/SRR16134765.lite.1", "size": "3079158949", "date": "2022-08-29 08:33:27", "md5": "172551c2cd3b6b7e1448f0850f3c76dc", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "39656986"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "11932801976"}}, "@attributes": {"alias": "GSM5606799_r7", "accession": "SRR16134765", "total_spots": "39656986", "total_bases": "11932801976", "size": "5416859031", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}, {"IDENTIFIERS": {"PRIMARY_ID": "SRR16134766", "SUBMITTER_ID": "GSM5606799_r8"}, "EXPERIMENT_REF": null, "RUN_ATTRIBUTES": {"RUN_ATTRIBUTE": {"TAG": "loader", "VALUE": "fastq-load.py", "UNITS": null}}, "Pool": {"Member": {"IDENTIFIERS": {"PRIMARY_ID": "SRS10387922", "EXTERNAL_ID": ["SAMN21924339", "GSM5606799"]}, "@attributes": {"member_name": "", "accession": "SRS10387922", "sample_name": "GSM5606799", "sample_title": "A673-EWS/FLI-Endo-HiC 2", "spots": "40127285", "bases": "12074045053", "tax_id": "9606", "organism": "Homo sapiens"}}}, "SRAFiles": {"SRAFile": [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L008_R1_001.fastq.gz", "size": "3611081689", "date": "2021-09-30 22:28:45", "md5": "892ee4c426ee787605c03cfd788a9221", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "A673_iLuc_197_Rep2_S4_L008_R2_001.fastq.gz", "size": "4183576789", "date": "2021-09-30 22:30:27", "md5": "2352af879afbfe51ac2c036471cbb1ee", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR16134766", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR16134766/SRR16134766", "size": "5456731633", "date": "2021-09-30 22:32:52", "md5": "6c690bffbd0a80f500f4724801815681", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR16134766.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR016/16134/SRR16134766/SRR16134766.lite.1", "size": "3115534225", "date": "2022-08-30 08:45:24", "md5": "726f911bdaf5e475afff15c9369918d3", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]}, "CloudFiles": {"CloudFile": [null, null, null, null]}, "Statistics": {"Read": [null, null], "@attributes": {"nreads": "2", "nspots": "40127285"}}, "Databases": {"Database": {"Table": {"Statistics": {"Rows": null, "Elements": null, "@attributes": {"source": "meta"}}, "@attributes": {"name": "SEQUENCE"}}}}, "Bases": {"Base": [null, null, null, null, null], "@attributes": {"cs_native": "false", "count": "12074045053"}}, "@attributes": {"alias": "GSM5606799_r8", "accession": "SRR16134766", "total_spots": "40127285", "total_bases": "12074045053", "size": "5456729137", "load_done": "true", "published": "2022-08-17 09:04:16", "is_public": "true", "cluster_name": "public", "has_taxanalysis": "1", "static_data_available": "1"}}]