EXPERIMENT_TITLE: GSM5702196: Cortisol; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Arima Genomics-HiC+ Kit Swift BiosciencesÒ Accel-NGSÒ 2S Plus DNA Library Kit Hi-C plus H3K27ac ChIP (HiChIP)
STUDY_DESCRIPTOR_STUDY_ABSTRACT: 30 B-lymphoblastoid cell lines (LCLs) with genome-wide genotype data were treated with hydrocortisone (GR agonist) and CORT108297 (GR modulator), followed by RNA-seq to identify PGx-eQTLs. We then integrated GR chromatin immunoprecipitation-sequencing (ChIP-seq) to characterize the epigenetic function of PGx-eQTLs that interfere with GR response elements. We also applied a high-throughput enhancer assay (STARR-seq) using PGx-eQTL loci DNA sequences to “scan” for drug-dependent SNPs. Overall design: H3K27ac HiChIP of LCL CA61 before and after Cortisol treatment
SAMPLE_TITLE: Cortisol
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "B lymphoblastoid cell line"}, {"TAG": "treatment", "VALUE": "100nM hydrocortisone, 8.5 hours"}, {"TAG": "cell line", "VALUE": "LCL CA61"}]
Pool_Member_@attributes_sample_title: Cortisol
RUN_SET_RUN_Pool_Member_@attributes_sample_title: Cortisol
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