EXPERIMENT_TITLE: GSM5328412: 4SPFC-F-J-085; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Crosslinked with 3% Formaldehyde-PBS, quenched with 1.1M Glycin (final concentration) after 3 minutes. Washed cell pellets with PBS. Subsequent HiC protocol steps were performed with the commercial ArimaKit HiC. Commercial Arima-HiC library.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Jurkat T cells have been fixated at different gravity conditions during the 4th Swiss Parabolic Flight Campaign (4SPFC). 1g inflight samples were generated by crosslinking 5 minutes prior to onset of the first parabola. Hypg samples were generated by crosslinking the samples at the end of the first hypergravity 1.8 g phase, 20 seconds after start of the parabola. µg/0g samples were generated by crosslinking the cells 20 seconds after the onset of microgravity during the first parabola. A ground control reference condition was generated by crosslinking cells that were stored inside the flight hardware but were not onboard during the flight. Overall design: 3 flight conditions (1gIF inflight control 5 minutes before first parabola, hypg hypergravity 1.8g during the first parabola, 0g microgravity during the first parabola), 1 ground control condition. 3 Samples per condition.
SAMPLE_TITLE: 4SPFC-F-J-085
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "ATCC Jurkat cells"}, {"TAG": "cell type", "VALUE": "Jurkat T cells"}, {"TAG": "condition", "VALUE": "hypg"}]
Pool_Member_@attributes_sample_title: 4SPFC-F-J-085
RUN_SET_RUN_Pool_Member_@attributes_sample_title: 4SPFC-F-J-085
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