EXPERIMENT_TITLE: GSM4712761: GSC1517_YY1_KD_rep1_Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: BL-Hi-C library construction were performed as described previously (Liang et al., 2017). PCR products between 300–700 bp were purified as BL-Hi-C library using Ampure XP beads and subjected to Illumina HiSeq X10 for paired end 150 bp sequencing.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Bridge Linker-Hi-C (BL-Hi-C) analysis after YY1 knockdown in glioblastoma stem cells Overall design: Bridge Linker-Hi-C (BL-Hi-C) analysis of chromatin interactions after YY1 knockdown in one glioblastoma stem cell (GSC1517)
SAMPLE_TITLE: GSC1517_YY1_KD_rep1_Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "patient-derived cells"}, {"TAG": "tissue", "VALUE": "glioblastoma stem cells"}, {"TAG": "cell type", "VALUE": "GSC1517"}, {"TAG": "treatment", "VALUE": "shYY1"}]
Pool_Member_@attributes_sample_title: GSC1517_YY1_KD_rep1_Hi-C
RUN_SET_RUN_Pool_Member_@attributes_sample_title: GSC1517_YY1_KD_rep1_Hi-C
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "YY1_KD_rep1_R1.fq.gz", "size": "7892138535", "date": "2020-08-06 17:20:44", "md5": "1b3a94f1b64facbdb01c1b2f4e14caf4", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "YY1_KD_rep1_R2.fq.gz", "size": "8875861287", "date": "2020-08-06 17:17:19", "md5": "5dc63407fc4a7fdf58be7416adf9408e", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR12403819", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR12403819/SRR12403819", "size": "11119225570", "date": "2020-08-06 17:27:02", "md5": "da50ed8aa2ddc789161c6ba21157d143", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR12403819.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos2/sra-pub-zq-18/SRR012/12403/SRR12403819/SRR12403819.lite.1", "size": "7539470790", "date": "2022-07-27 06:00:00", "md5": "5d302ce865109553e718ca00c6e453a6", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]