EXPERIMENT_TITLE: siARTD1+MyoD
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Trans-differentiation is the conversion of a differentiated somatic cell type into another one without de-differentiation and re-acquisition of the pluripotent state. Several studies suggested a role of ADP-ribosylation in regulating chromatin changes associated with differentiation and de-differentiation processes, but the role of ADP-ribosylation in cell-type conversion is unknown.MyoD is a master transcription factor, whose expression is sufficient to trans-differentiate fibroblasts to myoblasts. We observed a MyoD-dependent enhanced expression of ARTD1 that co-regulated only a subset of MyoD target genes in fibroblast-derived myoblasts. Comprehensive analysis of the genome organization by Hi-C analysis suggested that ARTD1 and ARTD1-dependent ADP-ribosylation might contribute to A/B compartmentalization in IMR90 cells, but not in a MyoD-dependent manner. The induction of ARTD1 was accompanied by the synthesis of nuclear ADP-ribosylation that was neither dependent on the cell cycle nor on DNA damage or transcriptional elongation. However, MyoD-induced trans-differentiation increased the DNA damage response induced by etoposide. Together, these data provide further insights into the molecular mechanisms associated with cell type conversion and nuclear ADP-ribosylation.
SAMPLE_TITLE: siARTD1+MyoD
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "strain", "VALUE": "IMR-90"}, {"TAG": "isolate", "VALUE": "Derived from lung tissue of a 16 week old female Caucasian foetus."}, {"TAG": "age", "VALUE": "16 weeks"}, {"TAG": "sex", "VALUE": "female"}, {"TAG": "tissue", "VALUE": "lung"}, {"TAG": "biomaterial_provider", "VALUE": "not applicable"}, {"TAG": "cell_line", "VALUE": "IMR-90"}, {"TAG": "cell_type", "VALUE": "lung fibroblast"}, {"TAG": "collected_by", "VALUE": "W.W. Nichols and associates"}, {"TAG": "disease", "VALUE": "Virus Susceptibility"}, {"TAG": "sample_type", "VALUE": "siARTD1"}, {"TAG": "treatment", "VALUE": "(+)Doxycycline"}, {"TAG": "BioSampleModel", "VALUE": "Human"}]
Pool_Member_@attributes_sample_title: siARTD1+MyoD
RUN_SET_RUN_Pool_Member_@attributes_sample_title: siARTD1+MyoD
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