EXPERIMENT_TITLE: GSM2496151: UMI-4C Primary Epidermal Keratinocyte Biological Replicate 1 - Differentiation Day 3 - EHF siRNA; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Cells were cross-linked in 2% formaldehyde for 10 min at RT and flash frozen.  Nuclei were digested with MboI and ligated in nuclei overnight. UMI-4C universal adapter was ligated and custom two-step bait primers were used for library PCR.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Genome-wide chromosome conformation capture (Hi-C) and promoter-capture Hi-C (CHi-C) were performed during epidermal differentiation. These data indicate that dynamic and constitutive enhancer-promoter contacts combine to control gene induction during differentiation and that chromosome conformation enables discovery of new TFs with distinct roles in this process. Overall design: Hi-C and CHi-C data from epidermal keratinocyte progenitor and differentiated cells.
SAMPLE_TITLE: UMI-4C Primary Epidermal Keratinocyte Biological Replicate 1 - Differentiation Day 3 - EHF siRNA
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Primary Epidermal Keratinocyte - Differentiation Day 3 - EHF siRNA"}, {"TAG": "cell type", "VALUE": "primary epidermal keratinocyte"}, {"TAG": "differentiation state", "VALUE": "Differentiated (Day 3)"}, {"TAG": "Sex", "VALUE": "male"}, {"TAG": "genotype/variation", "VALUE": "EHF knockdown"}]
Pool_Member_@attributes_sample_title: UMI-4C Primary Epidermal Keratinocyte Biological Replicate 1 - Differentiation Day 3 - EHF siRNA
RUN_SET_RUN_Pool_Member_@attributes_sample_title: UMI-4C Primary Epidermal Keratinocyte Biological Replicate 1 - Differentiation Day 3 - EHF siRNA
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