EXPERIMENT_TITLE: Hi-C sequencing data of HCT116 cells 1 hour after heat shock, replicate 1
STUDY_DESCRIPTOR_STUDY_ABSTRACT: The cell stress response is a multifaceted and evolutionarily conserved pathway aimed at inducing an anti-stress proteome. In eukaryotic organisms, the cascade of signals involved in this response has been significantly studied at the transcriptional, translational, and post-translational levels, including at the level of alternative polyadenylation (APA). APA can be regulated by DNA-methylation-sensitive chromatin looping. Herein, we examine the role of DNA methylation-regulated APA during the response to cell stress. We find that within the first several hours following exposure to stress, dynamic changes occur within the 3 regulatory region associated with APA of the candidate gene DNAJB6. Our results suggest that targeted DNA-demethylation occurs in a temporal manner following stress, promoting the expression of the DNAJB6 isoform associated with cytosolic chaperone activity. Furthermore, Hi-C data suggests that chromatin structural changes during stress are more widespread than previously appreciated.
SAMPLE_TITLE: 1 hour after heat shock replicate 1
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "isolate", "VALUE": "HCT 116 cell 1 hour after heat shock replicate 1"}, {"TAG": "age", "VALUE": "adult"}, {"TAG": "biomaterial_provider", "VALUE": "ATCC"}, {"TAG": "sex", "VALUE": "male"}, {"TAG": "tissue", "VALUE": "Large intestine; Colon"}, {"TAG": "cell_line", "VALUE": "HCT116"}, {"TAG": "treatment", "VALUE": "1 hour after heat shock"}, {"TAG": "BioSampleModel", "VALUE": "Human"}]
Pool_Member_@attributes_sample_title: 1 hour after heat shock replicate 1
RUN_SET_RUN_Pool_Member_@attributes_sample_title: 1 hour after heat shock replicate 1
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