EXPERIMENT_TITLE: GSM4872657: Hi-C_osteoblasts; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: The cross-linked cells were lysed in lysis buffer,fragmentation of the genome using restriction enzymes Hi-C libraries were prepared for sequencing using standard Illumina protocols
STUDY_DESCRIPTOR_STUDY_ABSTRACT: In this project, we use a simple digestion-ligation-only Hi-C (DLO Hi-C) technology, for whole-genome chromosome conformation capture, analysis of genes expression changes and transcriptional regulatory elements involved in BMMSC differentiation. Rely on GWAS data and eQTL analysis, our findings confirmed some previously reported genes that have an effect on OP, such as TIMP-2, MMP-2 and DAAM2, TMEM241 was less reported. Furthermore, our work provide a theoretical basis for the development of OP. Overall design: The bone marrow mesenchymal stem cells (BMMSCs), osteoblasts and adipocytes
SAMPLE_TITLE: Hi-C_osteoblasts
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "osteoblasts"}, {"TAG": "tissue", "VALUE": "bone"}, {"TAG": "cell type", "VALUE": "osteoblasts"}]
Pool_Member_@attributes_sample_title: Hi-C_osteoblasts
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