EXPERIMENT_TITLE: Illumina HiSeq 4000 paired end sequencing; Promoter capture Hi-C of human SGBS (Simpson-Golabi-Behmel syndrome preadipocyte) cells and iPSC-derived hypothalamic cells
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Human induced pluripotent stem cells (iPSCs) (Findiv 24382, Hutterites) were differentiated into hypothalamic arcuate-like neurons, as previously described by doi:10.1172/JCI79220 Hi-C libraries were prepared according to PMID: 29988018 Hi-C libraries were prepared according to PMID: 29988018
STUDY_DESCRIPTOR_STUDY_ABSTRACT: To identify distal chromatin contacts between promoters and their putative regulatory elements in SGBS preadipocytes and hypothalamic arcuate-like neurons derived from iPSC, we performed Hi-C with a capture step to enrich the library for contacts involving promoters. Hi-C with a capture step was performed according to PMID: 29988018
SAMPLE_TITLE: hypothalamic neurons
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "ENA first public", "VALUE": "2021-03-23"}, {"TAG": "ENA last update", "VALUE": "2021-03-10"}, {"TAG": "External Id", "VALUE": "SAMEA8228450"}, {"TAG": "INSDC center alias", "VALUE": "UNIVERSITY OF CHICAGO"}, {"TAG": "INSDC center name", "VALUE": "UNIVERSITY OF CHICAGO"}, {"TAG": "INSDC first public", "VALUE": "2021-03-23T08:54:54Z"}, {"TAG": "INSDC last update", "VALUE": "2021-03-10T19:48:55Z"}, {"TAG": "INSDC status", "VALUE": "public"}, {"TAG": "Submitter Id", "VALUE": "E-MTAB-10200:hypothalamic neurons"}, {"TAG": "broker name", "VALUE": "ArrayExpress"}, {"TAG": "cell line", "VALUE": "iPSC derived cell line"}, {"TAG": "cell type", "VALUE": "hypothalamic arcuate-like neurons"}, {"TAG": "common name", "VALUE": "human"}, {"TAG": "developmental stage", "VALUE": "embryo stage"}, {"TAG": "disease", "VALUE": "normal"}, {"TAG": "genotype", "VALUE": "wild type genotype"}, {"TAG": "organism part", "VALUE": "hypothalamus"}, {"TAG": "sample name", "VALUE": "E-MTAB-10200:hypothalamic neurons"}]
Pool_Member_@attributes_sample_title: hypothalamic neurons