EXPERIMENT_TITLE: GSM4332628: HUVECs, A-1-4_Hi-C; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Total RNA was isolated using TRIzol reagent (Ambion). Libraries from the resulting total RNA were prepared using the TruSeq paired-end mRNASeq kit. We used the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501) to prepare the ready-to-use DNA library for ATAC-Seq Hi-C library was performed as described in Belaghzal H et al, 2017.
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Tibetan's adaptation to high-altitude environment at the Qinghai-Tibetan plateau represents a remarkable case of natural selection during recent human evolution. We generated time series paired RNAseq, ATACseq and Hi-C data in Tibetan and Han Chinese's umbilical endothelial cells from normoxia to hypoxia condition. Our results provide a broad resource of genome-wide hypoxia regulatory network to characterize the effect of genetic variation in high-altitude adaptation, and indicates large-scale maps of variants need proper cell types to understand its act on gene regulation. Overall design: Time series paired RNAseq, ATACseq and Hi-C data in Tibetan and Han Chinese's umbilical endothelial cells from normoxia to hypoxia condition.
SAMPLE_TITLE: HUVECs, A-1-4_Hi-C
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Human Umbilical Vein Endothelial Cells"}, {"TAG": "cell type", "VALUE": "HUVEC"}, {"TAG": "individual", "VALUE": "Tibetan-1"}, {"TAG": "stress", "VALUE": "hypoxia"}, {"TAG": "time", "VALUE": "3d"}]
Pool_Member_@attributes_sample_title: HUVECs, A-1-4_Hi-C
RUN_SET_RUN_Pool_Member_@attributes_sample_title: HUVECs, A-1-4_Hi-C
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