EXPERIMENT_TITLE: GSM5822272: SKNMC_shGFP_HiC; Homo sapiens; Hi-C
EXPERIMENT_DESIGN_LIBRARY_DESCRIPTOR_LIBRARY_CONSTRUCTION_PROTOC: Genomic DNA was isolated from x-linked nuclei after Mbo1+HinfI restriction digestion, end filling with biotin-14ATP and following ligation, according to in-situ HiC protocol, and proceed to library construction. Followed Next Gen DNA Library Kit & Next Gen Indexing Kit from Active Motif
STUDY_DESCRIPTOR_STUDY_ABSTRACT: Tumor cell identity is the product of complex interactions between oncogenic drivers and mechanisms regulating normal differentiation pathways. Cell fate transitions observed in embryonic development involve structural changes in genomic organization that provide proper lineage specification, however, whether similar events also occur within tumor cells and contribute to cancer evolution remains largely unexplored. Here we modeled this process in the pediatric bone cancer Ewing sarcoma and investigated high resolution looping and large-scale 3D nuclear conformation changes associated with EWS-FLI1, the oncogenic fusion protein that drives this tumor. We find that chromatin interactions in Ewing sarcoma cells are dominated by highly connected looping hubs centered on EWS-FLI1 binding sites, which directly control the activity of linked enhancers and promoters to establish oncogenic expression programs. Depletion of EWS-FLI1 leads to the loss of looping networks associated with the fusion protein and, strikingly, also results in widespread nuclear reorganization through the establishment of new patterns of looping and large-scale inter-compartment connectivity that resemble the chromatin configuration of mesenchymal stem cells, a candidate cell of origin of this tumor. Our data thus demonstrate that major architectural features of nuclear organization in cancer cells can be linked to a single oncogenic event and readily reversed to re-establish latent differentiation programs. Overall design: Using in-situ HiC and H3K27ac Hi-ChIP, we profiled the organization and establishment of EWS-FLI1 mediated enhancer connectome in Ewing sarcoma model cells, A673 and SKNMC. The re-organization of enhancer network is further compared to the MSCs, a potential cell or origin or Ewing Sarcoma. Pooled HiC and Hi-ChIP libraries of each condition, shGFP/shFLI, from Ewing cells and wildtype MSCs were used in the study. NOTE FROM SUBMITTER: Since the MSC samples (HiC, HiChIP and ChIP-seq) are from patients, we did not include the raw data due to privacy concern.
SAMPLE_TITLE: SKNMC_shGFP_HiC
SAMPLE_SAMPLE_ATTRIBUTES_SAMPLE_ATTRIBUTE: [{"TAG": "source_name", "VALUE": "Ewing sarcoma cells"}, {"TAG": "cell type", "VALUE": "Ewing sarcoma cells"}, {"TAG": "treatment", "VALUE": "untreated"}]
Pool_Member_@attributes_sample_title: SKNMC_shGFP_HiC
RUN_SET_RUN_Pool_Member_@attributes_sample_title: SKNMC_shGFP_HiC
RUN_SET_RUN_SRAFiles_SRAFile: [{"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SKNMC_shGFP_HiC_R1.fastq", "size": "15297186108", "date": "2022-01-17 18:44:27", "md5": "ceb844d6376d4ab4ff62b58cf6638f40", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SKNMC_shGFP_HiC_R2.fastq", "size": "15299187882", "date": "2022-01-17 18:50:45", "md5": "26809de77e12aca27d1af911ec8ec6bd", "version": "1", "semantic_name": "fastq", "supertype": "Original", "sratoolkit": "0"}}, {"Alternatives": null, "@attributes": {"cluster": "public", "filename": "SRR17645664", "url": "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR17645664/SRR17645664", "size": "3094386195", "date": "2022-01-17 18:54:34", "md5": "d0a8c989820a3170ba24c70066216d96", "version": "1", "semantic_name": "SRA Normalized", "supertype": "Primary ETL", "sratoolkit": "1"}}, {"Alternatives": [null, null, null], "@attributes": {"cluster": "public", "filename": "SRR17645664.lite", "url": "https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos1/sra-pub-zq-38/SRR017/17645/SRR17645664/SRR17645664.lite.1", "size": "2379918759", "date": "2022-10-14 03:32:52", "md5": "5a9fd4c014becd80ddf5130150a9b209", "version": "1", "semantic_name": "SRA Lite", "supertype": "Primary ETL", "sratoolkit": "1"}}]