OpportunityTitle: Discovery, validation, and genotyping of single nucleotide polymorphisms in inconnu (Stenodus leucichthys).
AdditionalInformationOnEligibility: All potential applicants are eligible that meet the following requirements: 1.laboratory capacity to conduct next generation sequencing using a genome reduction technique such as restriction site associated DNA (RAD) tag sequencing 2. The computational capacity and ability to screen and analyze RAD tag sequence data to provide the Service with sufficient sequence for each polymorphism to develop SNP assays for the Life Technologies Quant Studio PCR system.
Description: This funding opportunity is for the discovery, validation and genotyping of approximately 10,000 single nucleotide polymorphisms, SNP, in 200 inconnu, Stenodus leucichthys, from Alaska. These SNPs will be used by the Service to address two objectives 1. perform mixed-stock analysis,MSA, on inconnu harvested in the winter subsistence fishery in the Hotham Inlet, Alaska, 2. describe and evaluate genetic population structure of inconnu throughout Alaska.
GrantorContactEmailDescription: Office
GrantorContactText: Jeffery Oslson, Conservation Genetics Laboratory, 907-786-3598&lt;br/&gt;jeffrey_olsen@fws.gov&lt;br/&gt;