FastQCFastQC Report
Tue 22 Jul 2025
iHEP_IgG_REP1_T1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameiHEP_IgG_REP1_T1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94628952
Total Bases13.4 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGGGG27563502.9127977661635733TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)