FastQCFastQC Report
Tue 22 Jul 2025
H1ESC_input_REP1_T1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH1ESC_input_REP1_T1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20455359
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTGGGGG6240723.0508973223105005TruSeq Adapter, Index 7 (97% over 36bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1297830.6344694317024698No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG734480.35906482990594296No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG622100.3041256816856649No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN494460.24172638573588465No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420270.20545716161715863No Hit
ATAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206640.10101998209857865No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)