FastQCFastQC Report
Tue 22 Jul 2025
H1ESC_IgG_REP1_T1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH1ESC_IgG_REP1_T1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108923868
Total Bases15.2 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG11802611.0835650823564216TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG7037450.6460888810889455TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG3067440.28161320896169423TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)