FastQCFastQC Report
Thu 17 Jul 2025
DE-3C_trimmed_2_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-3C_trimmed_2_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80137609
Total Bases10.9 Gbp
Sequences flagged as poor quality0
Sequence length20-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1658820.20699644283123045No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1538070.1919286111967728No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1027560.1282244395387439No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT996180.12430867509411218No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC995070.1241701633498948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)