FastQCFastQC Report
Thu 17 Jul 2025
DE-4_raw_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-4_raw_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96145572
Total Bases13.2 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG3253160.33835775609094093TruSeq Adapter, Index 13 (97% over 38bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2066560.2149407359082538No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1574060.16371632798648283No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1485230.15447721294954697No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1271180.13221409718171942No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1246320.12962843468235855No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1093730.1137577089873676No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT988130.10277436385733917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)