FastQCFastQC Report
Thu 17 Jul 2025
DE-3_raw_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-3_raw_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83123897
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTGGGGG3010140.3621269103877553TruSeq Adapter, Index 2 (97% over 37bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1801020.21666693514140706No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1304270.15690674367685142No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1181960.1421925634694437No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1038570.12494240976214097No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT942780.11341864782879466No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT883920.10633765161419224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)