FastQCFastQC Report
Thu 17 Jul 2025
DE-3C_raw_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-3C_raw_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80872082
Total Bases11.4 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5460000.6751402789407599No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1538170.19019789795939715No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1027590.12706362623383433No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT996260.12318960701419804No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC995120.12304864365925439No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN859970.10633706697448447No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)