FastQCFastQC Report
Thu 17 Jul 2025
DE-3C_raw_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-3C_raw_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80872082
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2132110.26363980588505187No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG2024840.250375648793115TruSeq Adapter, Index 9 (97% over 35bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1608400.19888198253632197No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1301280.16090596010623295No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1282420.1585738821463753No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1203230.14878187506041948No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG1167610.14437738847875833TruSeq Adapter, Index 7 (97% over 38bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1012450.12519153395852972No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT993850.12289160553576449No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG953110.1178540203775142No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG928430.11480228739504938No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA922950.11412467407479383No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG896500.11085407693596909No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA822950.10175946750078722No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC819870.10137861913830783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)