FastQCFastQC Report
Thu 17 Jul 2025
DE-2_raw_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-2_raw_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85186843
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGG3706680.43512353192851627TruSeq Adapter, Index 6 (97% over 36bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1511600.17744524233630773No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG991460.11638651757525514No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN953900.11197738599140244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)