FastQCFastQC Report
Thu 17 Jul 2025
DE-1_raw_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE-1_raw_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90005528
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length35-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTGGGGG8668820.9631430638349237TruSeq Adapter, Index 27 (97% over 39bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2444310.2715733193632284No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1674170.18600746389710643No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1544990.17165501212325535No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1328440.14759537880828832No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1231360.13680937464196644No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1096660.12184362720476458No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1066140.1184527243704409No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1060130.11778498760653901No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1003020.11143982178516856No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG994540.11049765743277457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)