---------------------------------------------------- ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/cutandrun vv3.1-g42502fb ---------------------------------------------------- Run Name: nasty_shannon #################################################### ## nf-core/cutandrun execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: 1. The full error message was: Error executing process > 'NFCORE_CUTANDRUN:CUTANDRUN:INPUT_CHECK:SAMPLESHEET_CHECK (sample_sheet.csv)' Caused by: Process `NFCORE_CUTANDRUN:CUTANDRUN:INPUT_CHECK:SAMPLESHEET_CHECK (sample_sheet.csv)` terminated with an error exit status (1) Command executed: check_samplesheet.py sample_sheet.csv samplesheet.valid.csv true cat <<-END_VERSIONS > versions.yml "NFCORE_CUTANDRUN:CUTANDRUN:INPUT_CHECK:SAMPLESHEET_CHECK": python: $(python --version | grep -E -o "([0-9]{1,}\.)+[0-9]{1,}") END_VERSIONS Command exit status: 1 Command output: ERROR: Please check samplesheet -> Control entry and sample entry must be different! Line: 'IgG,1,/data/abdenlab/share/4dn2csg/cut2run_batch2/CutnRun_H1_IgG_S1_L001_R1_001.fastq.gz,/data/abdenlab/share/4dn2csg/cut2run_batch2/CutnRun_H1_IgG_S1_L001_R2_001.fastq.gz,IgG' Command error: ERROR: Please check samplesheet -> Control entry and sample entry must be different! Line: 'IgG,1,/data/abdenlab/share/4dn2csg/cut2run_batch2/CutnRun_H1_IgG_S1_L001_R1_001.fastq.gz,/data/abdenlab/share/4dn2csg/cut2run_batch2/CutnRun_H1_IgG_S1_L001_R2_001.fastq.gz,IgG' Work dir: /home/gng/cutandrun_h1_batch2/work/3b/3382554f96db295f4053b2284f0383 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` The workflow was completed at 2023-05-26T17:39:39.216354-04:00 (duration: 1m 31s) The command used to launch the workflow was as follows: nextflow run nf-core/cutandrun -r 3.1 -profile docker --input /home/gng/cutandrun_h1_batch2/sample_sheet.csv --outdir /home/gng/cutandrun_h1_batch2/ --fasta /data/genomes/ncbi/GRCh38.analysisSet/GCA_000001405.15_GRCh38_no_alt_plus_hs38d1_analysis_set.fna.gz --bowtie2 /data/genomes/ncbi/GRCh38.analysisSet/GCA_000001405.15_GRCh38_no_alt_plus_hs38d1_analysis_set.fna.bowtie_index.tar.gz --gtf /data/genomes/ncbi/GRCh38.analysisSet/genes.gtf --peakcaller macs2 --skip_heatmaps true --max_memory 400.GB Pipeline Configuration: ----------------------- - revision: 3.1 - runName: nasty_shannon - containerEngine: docker - launchDir: /home/gng/cutandrun_h1_batch2 - workDir: /home/gng/cutandrun_h1_batch2/work - projectDir: /home/gng/.nextflow/assets/nf-core/cutandrun - userName: gng - profile: docker - configFiles: /home/gng/.nextflow/assets/nf-core/cutandrun/nextflow.config - input: /home/gng/cutandrun_h1_batch2/sample_sheet.csv - outdir: /home/gng/cutandrun_h1_batch2/ - bowtie2: /data/genomes/ncbi/GRCh38.analysisSet/GCA_000001405.15_GRCh38_no_alt_plus_hs38d1_analysis_set.fna.bowtie_index.tar.gz - gtf: /data/genomes/ncbi/GRCh38.analysisSet/genes.gtf - spikein_bowtie2: s3://ngi-igenomes/igenomes//Escherichia_coli_K_12_MG1655/NCBI/2001-10-15/Sequence/Bowtie2Index/ - spikein_fasta: s3://ngi-igenomes/igenomes//Escherichia_coli_K_12_MG1655/NCBI/2001-10-15/Sequence/WholeGenomeFasta/genome.fa - fasta: /data/genomes/ncbi/GRCh38.analysisSet/GCA_000001405.15_GRCh38_no_alt_plus_hs38d1_analysis_set.fna.gz - igenomes_base: s3://ngi-igenomes/igenomes/ - skip_heatmaps: true - minimum_alignment_q_score: 20 - normalisation_binsize: 50 - peakcaller: macs2 - max_memory: 400.GB - Date Started: 2023-05-26T17:38:08.009694-04:00 - Date Completed: 2023-05-26T17:39:39.216354-04:00 - Pipeline script file path: /home/gng/.nextflow/assets/nf-core/cutandrun/main.nf - Pipeline script hash ID: 933b3071ca6a81e9c2fc3c1dd6fa5968 - Pipeline repository Git URL: https://github.com/nf-core/cutandrun - Pipeline repository Git Commit: 42502fb44975e930eec865353c5481f472bcf766 - Pipeline Git branch/tag: 3.1 - Nextflow Version: 22.10.7 - Nextflow Build: 5853 - Nextflow Compile Timestamp: 18-02-2023 20:32 UTC -- nf-core/cutandrun https://github.com/nf-core/cutandrun